{"id":928,"date":"2024-10-30T04:01:41","date_gmt":"2024-10-30T04:01:41","guid":{"rendered":"https:\/\/alex-jimenez.com\/?post_type=rara-portfolio&p=928"},"modified":"2024-11-20T00:53:11","modified_gmt":"2024-11-20T00:53:11","slug":"single-cell-rnaseq-for-ulcerative-colitis","status":"publish","type":"rara-portfolio","link":"https:\/\/alex-jimenez.com\/?rara-portfolio=single-cell-rnaseq-for-ulcerative-colitis","title":{"rendered":"Single-Cell RNAseq For Ulcerative Colitis"},"content":{"rendered":"\n

Background<\/strong><\/p>\n\n\n\n

Single-cell RNA sequencing (scRNA-seq) is a cutting-edge technology that enables the analysis of gene expression at the level of individual cells. This method allows researchers to explore the heterogeneity within a population of cells, identifying distinct cell types, states, and responses that would be masked in bulk RNA sequencing. By isolating and sequencing RNA from thousands to millions of single cells, scRNA-seq provides a detailed map of cellular functions, developmental pathways, and disease mechanisms. It has revolutionized fields like developmental biology, cancer research, and immunology by uncovering rare cell populations and revealing how cells interact and evolve in complex biological systems. This technology has become an essential tool for understanding cellular diversity and dynamics across various tissues and conditions.<\/p>\n\n\n\n

In this workflow, Ulcerative Colitis patients were subjected to sc-RNAseq. Additional healthy controls were included. The main study design is summarized below<\/p>\n\n\n\n